Measuring deaminated nucleotide surveillance enzyme ITPA activity with an ATP-releasing nucleotide chimera
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چکیده
منابع مشابه
Measuring deaminated nucleotide surveillance enzyme ITPA activity with an ATP-releasing nucleotide chimera
Nucleotide quality surveillance enzymes play important roles in human health, by detecting damaged molecules in the nucleotide pool and deactivating them before they are incorporated into chromosomal DNA or adversely affect metabolism. In particular, deamination of adenine moiety in (deoxy)nucleoside triphosphates, resulting in formation of (d)ITP, can be deleterious, leading to DNA damage, mut...
متن کاملITPA protein, an enzyme that eliminates deaminated purine nucleoside triphosphates in cells.
Inosine triphosphate pyrophosphatase (ITPA protein) (EC 3.6.1.19) hydrolyzes deaminated purine nucleoside triphosphates, such as ITP and dITP, to their corresponding purine nucleoside monophosphate and pyrophosphate. In mammals, this enzyme is encoded by the Itpa gene. Using the Itpa gene-disrupted mouse as a model, we have elucidated the biological significance of the ITPA protein and its subs...
متن کاملThe nucleotide sequence of human acylamino acid-releasing enzyme.
The nucleotide sequence of a cDNA coding for the human acylamino acid-releasing enzyme (AARE, also known as acylpeptide hydrolase) [EC 3.4.19.1] subunit has been determined. The amino acid sequence of human AARE subunit deduced from its cDNA nucleotide sequence showed a high degree of identity (91.5%) with both the corresponding proteins from the pig and the rat. The AARE cDNA shows 99.2% ident...
متن کاملITPA (inosine triphosphate pyrophosphatase): from surveillance of nucleotide pools to human disease and pharmacogenetics.
Cellular nucleotide pools are often contaminated by base analog nucleotides which interfere with a plethora of biological reactions, from DNA and RNA synthesis to cellular signaling. An evolutionarily conserved inosine triphosphate pyrophosphatase (ITPA) removes the non-canonical purine (d)NTPs inosine triphosphate and xanthosine triphosphate by hydrolyzing them into their monophosphate form an...
متن کاملIntracellular ATP increases capsaicin-activated channel activity by interacting with nucleotide-binding domains.
Capsaicin (CAP)-activated ion channel plays a key role in generating nociceptive neural signals in sensory neurons. Here we present evidence that intracellular ATP upregulates the activity of capsaicin receptor channel. In inside-out membrane patches isolated from sensory neurons, application of CAP activated a nonselective cation channel (i(cap)). Further addition of ATP to the bath caused a s...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2017
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkx774